- #How to determine peak purity in agilent chemstation how to
- #How to determine peak purity in agilent chemstation software
Posting is currently disabled in this topic. Agilent does not offer one, but we know many scientists whom have taken the one offered by.
#How to determine peak purity in agilent chemstation software
A peak witha Puritu Factor 99.985 it could be considered as a not pure peak.Īny related document ? (USP doesn't specify the specific system to evaluate a peak purity). BTW: Before you use the feature you should have a great deal of experience using ChemStation's Data analysis software (advanced integration tools, spectral and file overlays plus a good understanding of all of the peak purity tools in the menu). Related with Chemstation Software, sometimes I had problems with the thereshold value if it's automatically calculated by the system. The peak impurity check, or peak purity is a 3D-UV feature in OpenLAB CDS allowing you to verify if the peaks can be considered as pure (no impurity underneath), or impure if there is any impurity across the peak absorbing at the defined UV wavelengths. well-isolated colonies to serve as a check for purity. When I said "official" method I was thinking on something that would be accepted by the FDA or any other Health Authority when you develop a method and want to prove that the method is Stability Indicating and Suitable for Stability studies. Packard/Compaq computer with Agilent ChemStation software (version B.04.03 or above) installed is. A Dell or Hewlett-Packard/Compaq computer with Agilent ChemStation software (version A.10. The 6890 GCs can be dual or single tower. Back when we used ChemStation I had the software automatically calculate the threshold value. or 27-vial turret (see Figure 1-2) or an Agilent 7890 GC with automatic liquid sampler, injector and 100-vial tray. I believe with ChemStation and the 1100 DAD you can have the software automatically calculate a threshold value base on signal to noise or you can manually set a threshold value. What do you mean by "official", from agilent, or from the FDA, EPA, etc.?
We work with an Agilent 1100 DAD, and we could do a Peak Purity.Īny related document would be appreciated Agilent ChemStation ed.
#How to determine peak purity in agilent chemstation how to
Is there any official document to know how to fix a Peak Purity criteria to confirm the peak purity in a HPLC Assay ? I'm not sure if the OP is still interested, but if so I'll try to post anything else I remember.Chromatography Forum: LC Archives: HPLC PEAK PURITY I do know the macro (enh_grph.mac) switches units at some point (it divides by 60,000, which gets you ms from minutes). the small differnce there is may have to do with rounding that happens when you change from scans or minutes to seconds. I can't remember if I was ever able to replicate the calculations it does exactly. I think I remember seeing a note in the macro that led me to believe that the 100 was just a fudge factor to get more manageable numbers. The Chemstation one seems to use counts * milliseconds / 100. I think that it really does use that simple equation. I think the RTE integrator measures area in units of counts*seconds. I did a dive into the macros (shudder) a while ago because I wanted to switch to the latter, but wanted to understand that switch before I made it. It depends if you use the Chemstation integrator or the RTE integrator. Verify the peak purity for all injections, look at the Result Window graphics 11. Look at the Purity tab of the Processing Method.
Save Processing Method in the Method Set 9. I wish instrument manufacturers made it easy to figure out what math their software is using! For Peak Purity use interval time between 8 and 10 min.